High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus)

Roberto Méndez-Pérez, Rodrigo García-López, J. Santiago Bautista-López Bautista-López, Jorge Vázquez-Castellanos, Carlos Alvarez-González, Emyr Peña-Marín, Eduardo Baltierra-Trejo, Randy Adams-Schroeder, Verónica Domínguez-Rodríguez, Carolina Melgar-Valdés, Rafael Martínez-García, Andrés Moya, Rodolfo Gómez-Cruz

Submited: 2019-08-02 16:25:38 | Published: 2020-07-06 19:40:47

DOI: https://doi.org/10.3856/vol48-issue3-fulltext-2419

Abstract


Tropical gar (Atractosteus tropicus) is freshwater and estuarine fish, inhabiting the Earth since the Mesozoic era and undergoing limited physiological variation ever since. Besides its recognized cultural and
scientific relevance, the species has seen remarkable growth in its economic impact due to pisciculture. In this study, we present the first report of the whole taxonomic composition of microbial communities in gut contents in juveniles and adults of A. tropicus, by sex and origin (wild and cultivated). For this study, 508 genera were identified, with the most and least abundant being Cetobacterium and Paludibacter, respectively. Fusobacteria, Proteobacteria, Firmicutes, and Bacteroidetes phyla are the core gut microbiome of A. tropicus juvenile and adult by sex and origin. Deinococcus-Thermus phylum sequence was only identified in wild-type males. In the phylogenetic trees reconstruction Lactococcus lactis strains CAU929 and CAU6600, Cp6 and CAU9951,
Cetobacterium strain H69, Aeromonas hydrophila strain P5 and WR-5-3-2, Aeromonas sobria strain CP DC28 and Aeromonas hydrophila were identified, some of them with probiotic potential within the three dominant phyla in core gut microbiome in A. tropicus adults, especially in wild-type organisms. Myroides genus was recognized in microbiota gut of the cultivated juvenile A. tropicus. Nevertheless, Alpha diversity indicated that the highest gut microbiota abundance and richness is found in cultivated juvenile and wild-type adult A. tropicus female, rather than adult wild-type males and the least gut microbiota abundance and richness is found in a cultivated adult of A. tropicus for both sexes.


Méndez-Pérez R, García-López R, Bautista-López J, Vázquez-Castellanos J, Alvarez-González C, Peña-Marín E, Baltierra-Trejo E, Adams-Schroeder R, Domínguez-Rodríguez V, Melgar-Valdés C, Martínez-García R, Moya A, Gómez-Cruz R. High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus). Lat. Am. J. Aquat. Res.. 2020;48(3): 456-479. Available from: doi:10.3856/vol48-issue3-fulltext-2419 [Accessed 13 Aug. 2020].
Méndez-Pérez, R., García-López, R., Bautista-López, J., Vázquez-Castellanos, J., Alvarez-González, C., Peña-Marín, E., Baltierra-Trejo, E., Adams-Schroeder, R., Domínguez-Rodríguez, V., Melgar-Valdés, C., Martínez-García, R., Moya, A., & Gómez-Cruz, R. (2020). High-throughput sequencing of the 16S rRNA gene to analyze the gut microbiome in juvenile and adult tropical gar (Atractosteus tropicus). Latin American Journal of Aquatic Research, 48(3), 456-479. doi:http://dx.doi.org/10.3856/vol48-issue3-fulltext-2419