Redox balance and tissue development of juvenile Piaractus mesopotamicus subjected to high stocking density and fed dry diets containing nutraceutical food

Debora B. Moretti, Wiolene M. Nordi, Raul Machado-Neto

Submited: 2018-08-13 12:51:05 | Published: 2019-07-08 12:39:11

DOI: https://doi.org/10.3856/vol47-issue3-fulltext-4

Abstract


Colostrum is a source of molecules with nutraceutical features that can attenuate the consequences of adverse conditions, such as densification. Thus, the redox balance and tissue development of juvenile Piaractus mesopotamicus subjected to high stocking density and fed diets containing lyophilized bovine colostrum (LBC) were evaluated. Juveniles were distributed into 16 cages making 50 kg fish m-3 and fed diets containing 0, 10, 20 and 30% inclusion of LBC (n = 4). After 30 days, enteric, hepatic, muscular, renal and branchial tissue were sampled for the determination of glutathione peroxidase (GPx), superoxide dismutase (SOD) and catalase activity, oxygen radical absorbance capacity (ORAC), lipid peroxidation and protein and nucleotides content. SOD activity and DNA content were higher in 20% LBC compared to the others in the enteric tissue (P < 0.10). ORAC value in erythrocytes was higher in 30% LBC than 0 and 10% LBC (P < 0.10). Juveniles fed 10% LBC showed higher lipid peroxidation in liver than 0 and 20% LBC (P < 0.10) and those fed 20% LBC showed lower protein/RNA ratio compared to the others (P < 0.10). Bovine colostrum determined a decrease in liver protein synthesis and increased in intestinal DNA content with an intake of 20% of this milk secretion. Additionally, LBC increased the protection of the enteric tissue of juveniles from superoxide radicals and blood antioxidant capacity. We conclude that bovine colostrum can be used as a nutraceutical food for fish, with positive effect in redox balance and tissue development in high concentrations of inclusion in the diet, 20 and 30%.


Moretti D, Nordi W, Machado-Neto R. Redox balance and tissue development of juvenile Piaractus mesopotamicus subjected to high stocking density and fed dry diets containing nutraceutical food. Lat. Am. J. Aquat. Res.. 2019;47(3): 423-432. Available from: doi:10.3856/vol47-issue3-fulltext-4 [Accessed 17 Jul. 2019].
Moretti, D., Nordi, W., & Machado-Neto, R. (2019). Redox balance and tissue development of juvenile Piaractus mesopotamicus subjected to high stocking density and fed dry diets containing nutraceutical food. Latin American Journal of Aquatic Research, 47(3), 423-432. doi:http://dx.doi.org/10.3856/vol47-issue3-fulltext-4