Contributions to the nutrition of the American crocodile Crocodylus acutus (Cuvier, 1807) in captivity

Pablo S. Hernández-Hurtado, Héctor Nolasco-Soria, Olimpia Carrillo-Farnés, Helios Hernández-Hurtado, Rafael García de Quevedo-Machain, Gustavo Casas-Andreu, Cynthia Montoya-Martínez, Fernando Vega-Villasante

Submited: 2017-01-26 15:30:14 | Published: 2018-03-15 17:16:05

DOI: https://doi.org/10.3856/vol46-issue1-fulltext-3

Abstract


In this work we evaluated the essential amino acid profile of muscle Crocodylus acutus and calculated their chemical score of main proteins used in their diet in captivity. The separation and identification of amino acids was carried out by high performance liquid chromatography coupled to a fluorescence detector. The calculation of chemical score was obtained by dividing the value of each essential amino acid between the same amino acid of the reference protein. The lowest value of the relationships is the chemical score. The amino acids present in greater quantities in the tail muscle are glutamic acid, lysine and leucine (16.96, 9.84 and 8.87 g amino acid per 100 g of protein, respectively), whereas histidine, methionine and tryptophan (2.99, 2.93 and 0.59 g amino acid per 100 g of protein) were the lowest. The chemical score obtained results showed that the proteins of animal origin, preferably marine fish and supplemented with terrestrial animals ingredients, including beef liver appear to be the most effective. The incorporation of plant proteins in diets for C. acutus does not appear as a viable alternative due to deficiencies in several essential amino acids such as methionine, lysine and threonine. amino acids, chemical score, protein, crocodile, feeding, diet.


Hernández-Hurtado P, Nolasco-Soria H, Carrillo-Farnés O, Hernández-Hurtado H, García de Quevedo-Machain R, Casas-Andreu G, Montoya-Martínez C, Vega-Villasante F. Contributions to the nutrition of the American crocodile Crocodylus acutus (Cuvier, 1807) in captivity. Lat. Am. J. Aquat. Res.. 2018;46(1): 15-19. Available from: doi:10.3856/vol46-issue1-fulltext-3 [Accessed 22 Apr. 2019].
Hernández-Hurtado, P., Nolasco-Soria, H., Carrillo-Farnés, O., Hernández-Hurtado, H., García de Quevedo-Machain, R., Casas-Andreu, G., Montoya-Martínez, C., & Vega-Villasante, F. (2018). Contributions to the nutrition of the American crocodile Crocodylus acutus (Cuvier, 1807) in captivity. Latin American Journal of Aquatic Research, 46(1), 15-19. doi:http://dx.doi.org/10.3856/vol46-issue1-fulltext-3