Protocol for obtaining rainbow trout (Oncorhynchus mykiss) axenic fingerlings

María Fernanda Jiménez-Reyes, Gabriel Yany, Jaime Romero

Submited: 2016-05-19 17:31:23 | Published: 2018-03-15 16:44:48

DOI: https://doi.org/10.3856/vol45-issue5-fulltext-22

Abstract


In this paper a protocol based on the application of Buffodine® disinfectant and a mix of antibacterials for obtaining rainbow trout (Oncorhynchus mykiss) axenic fingerlings is described. This allowed to obtain and maintain axenic trout fingerlings for a period of 26 days. This time allow to achieve a gnotobiotic organism, a fundamental tool in studies of the understanding bacteria-host interaction or host-parasite interaction. 


Jiménez-Reyes M, Yany G, Romero J. Protocol for obtaining rainbow trout (Oncorhynchus mykiss) axenic fingerlings. Lat. Am. J. Aquat. Res.. 2018;45(5): 1064-1069. Available from: doi:10.3856/vol45-issue5-fulltext-22 [Accessed 18 Feb. 2020].
Jiménez-Reyes, M., Yany, G., & Romero, J. (2018). Protocol for obtaining rainbow trout (Oncorhynchus mykiss) axenic fingerlings. Latin American Journal of Aquatic Research, 45(5), 1064-1069. doi:http://dx.doi.org/10.3856/vol45-issue5-fulltext-22