Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP-B)

Martín Rodrigo Acedo-Valdez, José Manuel Grijalva-Chon, Eduardo Larios-Rodríguez, Amir Darío Maldonado-Arce, Fernando Mendoza-Cano, José Arturo Sánchez-Paz, Reina Castro-Longoria

Submited: 2016-05-25 19:19:08 | Published: 2017-05-22 18:01:37

DOI: https://doi.org/10.3856/vol45-issue2-fulltext-17

Abstract


The necrotizing hepatopancreatitis is caused by a gram-negative Rickettsia-like and pleomorphic bacterium that infects shrimp. It is well known that silver is an effective bactericidal but experiments with aquatic organisms are scarce, even more at nanoparticle level. The hypothesis of this paper is that the administration of silver nanoparticles (AgNPs) reaches the host cells acting as a bactericide against this intracellular bacterium. For this experiment AgNO3 was used as precursor of silver, and extract of dried leaves of green tea Camellia sinensis and neem tree Azadirachta indica as reducing agents. Different lots of infected shrimp received 0, 5 and 35 μg of AgNP by forced feeding. The differences between the amount of bacterial nodules in shrimp hepatopancreas and shrimp mortality relative to the amount of AgNP demonstrated to be effective against this bacterium.

Acedo-Valdez M, Grijalva-Chon J, Larios-Rodríguez E, Maldonado-Arce A, Mendoza-Cano F, Sánchez-Paz J, Castro-Longoria R. Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP-B). Lat. Am. J. Aquat. Res.. 2017;45(2). Available from: doi:10.3856/vol45-issue2-fulltext-17 [Accessed 22 Jan. 2019].
Acedo-Valdez, M., Grijalva-Chon, J., Larios-Rodríguez, E., Maldonado-Arce, A., Mendoza-Cano, F., Sánchez-Paz, J., & Castro-Longoria, R. (2017). Antibacterial effect of biosynthesized silver nanoparticles in Pacific white shrimp Litopenaeus vannamei (Boone) infected with necrotizing hepatopancreatitis bacterium (NHP-B). Latin American Journal of Aquatic Research, 45(2). doi:http://dx.doi.org/10.3856/vol45-issue2-fulltext-17