Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock

Silvano García, George S. Yasui, Jurandir J. Bernardes-Júnior, Bruno Corrêa da Silva, Hilton Amaral-Júnior, Evoy Zaniboni-Filho

Submited: 2015-09-01 15:15:05 | Published: 2017-03-09 09:32:20

DOI: https://doi.org/10.3856/vol45-issue1-fulltext-22

Abstract


This study evaluated sequential double-temperature shock (heat and cold) to induce triploidy in Rhamdia quelen. Fertilized eggs were heat shocked at 37, 39 and 41°C at 2 min post-fertilization and then cold shocked at 1°C for 20 min. Intact eggs were used as controls. Egg viability in control groups was higher (65.5 ± 37.0%) than that at 37°C (58.2 ± 37.7%), 39°C (1.8 ± 0.3%) and 41ºC (0%). The survival of control hatchery (64.6 ± 36.8%) was higher compared to treatments at 37ºC (24.4 ± 15.5%) and 39ºC (0.6 ± 0.07%). Triploid percentages were 98.5% at 37°C and 100% at 39°C. Therefore, while double-temperature shock induced 100% triploidy in R. quelen, it also significantly decreased embryo survival and increased the deformity larvae


García S, Yasui G, Bernardes-Júnior J, Corrêa da Silva B, Amaral-Júnior H, Zaniboni-Filho E. Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock. Lat. Am. J. Aquat. Res.. 2017;45(1): 209-212. Available from: doi:10.3856/vol45-issue1-fulltext-22 [Accessed 16 Sep. 2019].
García, S., Yasui, G., Bernardes-Júnior, J., Corrêa da Silva, B., Amaral-Júnior, H., & Zaniboni-Filho, E. (2017). Induction of triploidy in Rhamdia quelen (Siluriformes, Heptapteridae) by double-temperature shock. Latin American Journal of Aquatic Research, 45(1), 209-212. doi:http://dx.doi.org/10.3856/vol45-issue1-fulltext-22