Analysis of repeated compound units in ORF94 of white spot syndrome virus isolated from Litopenaeus vannamei from outbreak and non-outbreak shrimp farms in Sonora, Mexico

Libia Z. Rodríguez-Anaya, José Cuauhtémoc Ibarra-Gámez, Fernando Lares-Villa, Ramon Casillas-Hernández, Alejandro Sánchez-Flores, José Reyes González-Galaviz


DOI: https://doi.org/10.3856/vol44-issue4-fulltext-21

Abstract


White spot syndrome virus (WSSV) is the viral pathogen with the most negative impact on shrimp farming. In Sonora, Litopenaeus vannamei culture has decreased 50% during 2010-2012 due to WSSV outbreaks. The ORF94 has proven to be most useful for the analysis of WSSV variability. Several studies have suggested a correlation between its Repeat Units (RUs) and WSSV virulence as follows: the fewer RUs (<9) the higher mortality rate. In order to support this, we analyzed shrimps from eight farm periods and identified the WSSV-variety present in each one. In outbreaks, the presence of <8 RUs with a dominance of 3 RUs was notable in the last four years. Although it is still not clear how host-virus interactions and pond´s environment affect the transition of the infection just from the presence of the virus in shrimps to an outbreak, these results are a step forward in understanding the pond status and ways of predicting the likelihood of a WSSV infection becoming an outbreak.


Rodríguez-Anaya L, Ibarra-Gámez J, Lares-Villa F, Casillas-Hernández R, Sánchez-Flores A, Reyes González-Galaviz J. Analysis of repeated compound units in ORF94 of white spot syndrome virus isolated from Litopenaeus vannamei from outbreak and non-outbreak shrimp farms in Sonora, Mexico. Lat. Am. J. Aquat. Res.. 2017;44(4): 850-854. Available from: doi:10.3856/vol44-issue4-fulltext-21 [Accessed 17 Oct. 2019].
Rodríguez-Anaya, L., Ibarra-Gámez, J., Lares-Villa, F., Casillas-Hernández, R., Sánchez-Flores, A., & Reyes González-Galaviz, J. (2017). Analysis of repeated compound units in ORF94 of white spot syndrome virus isolated from Litopenaeus vannamei from outbreak and non-outbreak shrimp farms in Sonora, Mexico. Latin American Journal of Aquatic Research, 44(4), 850-854. doi:http://dx.doi.org/10.3856/vol44-issue4-fulltext-21