Effects of dietary mannan oligosaccharide on the growth, survival, intestinal morphometry and nonspecific immune response for Siamese fighting fish (Betta splendens Regan, 1910) larvae

Rafael Vieira de Azevedo, Dayane Kelly da Silva Azevedo, João Marcos dos Santos Júnior, João Carlos Fosse Filho, Dalcio Ricardo de Andrade, Luís Gustavo Tavares Braga, Manuel Vazquez Vidal Júnior


DOI: https://doi.org/10.3856/vol44-issue4-fulltext-15

Abstract


A 28-day experiment was carried out to evaluate the effect of dietary prebiotic mannan oligosaccharide (MOS) on the growth, survival, intestinal morphometry and nonspecific immune response of the Siamese fighting fish (Betta splendens Regan, 1910) larvae. The MOS supplementation levels evaluated were: 0; 2; 4 and 6 g kg-1. Two hundred and forty larvae (4.21 ± 0.26 mm) were distributed into 16 plastic aquaria (3 L) at a density of 5 larvae L-1. All larvae received live food for seven days, followed by a period of eight days of co-feeding (live food + inert diet) and, finally, 13 days of inert diet. At the end of feeding-trial, there were no significant differences between growth, survival, intestinal morphometry and alkaline phosphatase activity of larvae fed the control or MOS supplemented diets (P > 0.05). However, the lysozyme activity in larvae fed 2 g MOS kg-1 was significantly higher compared to other treatments (P = 0.0001). These results revealed that dietary MOS can be considered as a beneficial immunostimulant supplement for B. splendens larvae.

de Azevedo R, Azevedo D, dos Santos Júnior J, Fosse Filho J, de Andrade D, Braga L, Vidal Júnior M. Effects of dietary mannan oligosaccharide on the growth, survival, intestinal morphometry and nonspecific immune response for Siamese fighting fish (Betta splendens Regan, 1910) larvae. Lat. Am. J. Aquat. Res.. 2017;44(4). Available from: doi:10.3856/vol44-issue4-fulltext-15 [Accessed 20 Jan. 2019].
de Azevedo, R., Azevedo, D., dos Santos Júnior, J., Fosse Filho, J., de Andrade, D., Braga, L., & Vidal Júnior, M. (2017). Effects of dietary mannan oligosaccharide on the growth, survival, intestinal morphometry and nonspecific immune response for Siamese fighting fish (Betta splendens Regan, 1910) larvae. Latin American Journal of Aquatic Research, 44(4). doi:http://dx.doi.org/10.3856/vol44-issue4-fulltext-15