Effect of LHRHa on the reproductive performance of spotted sand bass Paralabrax maculatofasciatus (Steindachner, 1868).

Juan Pablo Alcántar, Hugo Skyol Pliego, Silvie Dumas, Renato Peña, Martín Oscar Rosales, Pablo Pintos


DOI: https://doi.org/10.3856/vol44-issue3-fulltext-7

Abstract


Hormonal induction was investigated in order to induce and synchronize the spawning of spotted sand bass (Paralabrax maculatofasciatus). We conducted three experiments with wild fish captured in the Gulf of California: 1) Spawning induction with LHRHa, 2) LHRHa effects on motility and sperm cell density (both experiments using LHRHa concentrations of 25, 50, 100 µg kg-1 and saline solution), 3) Induction and incubation of spawns obtained with LHRHa, using concentrations of 12.5, 25, 50 µg kg-1 and saline solution. In experiment 1, a higher spawning ratio was observed in the concentration of 25 µg kg-1; however, no significant differences were observed in the proportion of floating (viable) eggs. In experiment 2, significant differences (P < 0.05) were observed in duration of sperm motility, with highest duration registered in the wild control group. The density of sperm cells also showed significant differences (P < 0.05), with the laboratory control group and concentration of 12.5 µg kg-1 showing the higher densities. In experiment 3, significant differences (P < 0.05) were observed between treatments in the volume of spawned eggs and the proportion of fertilized eggs, with the highest values shown in the concentrations of 50 and 12.5 µg kg-1, respectively. Survival at hatching and proportion of live yolk-sac larvae were significantly higher (P < 0.05) in the concentration of 12.5 µg kg-1. In this work, LHRHa was effective in inducing and synchronizing the spawning of spotted sand bass, the product of which will be used for massive egg production. 


Alcántar J, Pliego H, Dumas S, Peña R, Rosales M, Pintos P. Effect of LHRHa on the reproductive performance of spotted sand bass Paralabrax maculatofasciatus (Steindachner, 1868).. Lat. Am. J. Aquat. Res.. 2017;44(3). Available from: doi:10.3856/vol44-issue3-fulltext-7 [Accessed 24 Jan. 2019].
Alcántar, J., Pliego, H., Dumas, S., Peña, R., Rosales, M., & Pintos, P. (2017). Effect of LHRHa on the reproductive performance of spotted sand bass Paralabrax maculatofasciatus (Steindachner, 1868).. Latin American Journal of Aquatic Research, 44(3). doi:http://dx.doi.org/10.3856/vol44-issue3-fulltext-7