Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus (Palaemonidae)

TIAGO VIANA DA COSTA, Lidia Miyako Yoshii Oshiro, LAURA SUSANA LOPEZ-GRECO, Emanuela Paula Melo, Luciana Antunes de Mattos, Andrea Bambozzi


DOI: https://doi.org/10.3856/vol44-issue2-fulltext-24

The freshwater prawns are distributed throughout the Americas including Brazil. M. acanthurus is caught and consumed mainly by traditional populations and besides that, it is a species with potential for aquaculture because of its size. Studies on this species reproduction are poorly developed being necessary for culture. The purpose of this study was to identify the best voltage for spermatophores extraction and smaller sizes producing spermatophores. Two voltages were assayed (4.5 and 6.0 volts) with sperm counts made ​​based on sperm survival after eosin-nigrosin staining; the production by the right and left halves of each spermatophore were also compared. Prawns were also separated into class intervals and the productions of spermatophores and sperm survival were analyzed. At the end of the experiments, 6.0 volts was considered the best voltage for seminal material removal due to lower mortality and animals weighting at least 5.0 g and 18.00 mm of total length could be used for reproduction studies.    

COSTA T, Oshiro L, LOPEZ-GRECO L, Melo E, Mattos L, Bambozzi A. Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus (Palaemonidae). Lat. Am. J. Aquat. Res.. 2017;44(2). Available from: doi:10.3856/vol44-issue2-fulltext-24 [Accessed 24 Jan. 2019].
COSTA, T., Oshiro, L., LOPEZ-GRECO, L., Melo, E., Mattos, L., & Bambozzi, A. (2017). Determination of adequate voltage and animal size for the extraction of spermatophores in the freshwater prawn Macrobrachium acanthurus (Palaemonidae). Latin American Journal of Aquatic Research, 44(2). doi:http://dx.doi.org/10.3856/vol44-issue2-fulltext-24