Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms

Victor M. Aguilera Ramos


DOI: https://doi.org/10.3856/vol43-issue4-fulltext-20

Abstract


Recent field and experimental evidence collected in southern upwelling region of the Humboldt Current showed a rapid decline (72 h) in the egg production rate (EPR) of copepods when they were fed steadily and solely with the local bloom-forming diatom Thalassiosira rotula.  This phenotypic outcome may be associated to changes in the structure of protein profiles. This hypothesis was tested through the comparison of the structure of protein profiles (i.e., number and intensity of electrophoretic bands) elaborated with adult and eggs of the mid-sized copepod Calanoides patagoniensis assessing two food conditions leading to different EPR: T. rotula (low) and Prorocentrum minimum (high). The structure of protein profiles resulted similar mostly at banding region of 100 to 89-kDa where nutritional complexes have been previously found.  However, the band at 56-kDa, where different stress-related proteins haven been found, was only observed with T. rotula. Since egg viability was independent of food treatments and thus, embryos nutritional requirements should have been satisfied; current findings suggest us that the EPR decline may have obeyed to post-digestive processes associated to the sustained diatoms-based diet. Thus, implications for copepod reproductive performance under intense spring-summer diatoms bloom occurring in the study area are discussed.

Aguilera Ramos V. Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms. Lat. Am. J. Aquat. Res.. 2017;43(4). Available from: doi:10.3856/vol43-issue4-fulltext-20 [Accessed 22 Jan. 2019].
Aguilera Ramos, V. (2017). Electrophoretic protein profiles of mid-sized copepod Calanoides patagoniensis steadily fed bloom-forming diatoms. Latin American Journal of Aquatic Research, 43(4). doi:http://dx.doi.org/10.3856/vol43-issue4-fulltext-20