Bacillus cereus var. toyoi and Bacillus subtilis C-3102 for Nile tilapia juveniles in cages

Nilton Garcia Marengoni, Milton Cézar Moura, Newton Tavares Escocard Oliveira, Robie Allan Bombardelli, Daniele Menezes Albuquerque


DOI: https://doi.org/10.3856/vol43-issue3-fulltext-23

Abstract


This study aimed at evaluating the gut colonization by probiotics, the influence on the bacterial microflora, hematological profile, fish performance, rates body and proximate composition and water quality parameters in the cultivation of Nile tilapia juvenile of the GIFT strain. A total of 1800 fry were randomly distributed into four experimental groups (T1 = basal diet (BD) without probiotic addition, T2 = BD + 0.5 % Bacillus cereus var. toyoi (BC), T3 = BD + 0.5% Bacillus subtilis C-3102 (BS); T4 = BD + 0.25 % BC + 0.25 % BS) with five replicates for 127 days. Cages were used with volumes of 0.175 m3, containing 90 tilapia fry with average weight of 0.34 ± 0.06 g, installed in individual ponds with 8.4 m3 of water. Feed conversion ranged from 1.69 ± 0.29 to 1.96 ± 0.94 and the survival from 80 ± 18% to 90 ± 2%, respectively for groups containing B. subtilis and without probiotic. It was not observed significant difference (P > 0.05) of the values ​​of RBC, WBC, MCH, MCHC, RDW, lymphocytes, eosinophils, monocytes, platelets and MPV among the experimental groups evaluated. The addition of probiotics B. cereus var. toyoi and B. subtilis C-3102 used individually and combined colonized the intestine of fish; however, without influencing negatively (P > 0.05) on the intestinal bacterial microflora, as well as on the growth performance, body composition rate and water quality parameters. The dietary probiotic influences the hematological profile, especially the hemoglobin, hematocrit, glucose and neutrophils in Nile tilapia juveniles.  


Marengoni N, Moura M, Oliveira N, Bombardelli R, Albuquerque D. Bacillus cereus var. toyoi and Bacillus subtilis C-3102 for Nile tilapia juveniles in cages. Lat. Am. J. Aquat. Res.. 2017;43(3). Available from: doi:10.3856/vol43-issue3-fulltext-23 [Accessed 20 Jan. 2019].
Marengoni, N., Moura, M., Oliveira, N., Bombardelli, R., & Albuquerque, D. (2017). Bacillus cereus var. toyoi and Bacillus subtilis C-3102 for Nile tilapia juveniles in cages. Latin American Journal of Aquatic Research, 43(3). doi:http://dx.doi.org/10.3856/vol43-issue3-fulltext-23