MOLECULAR CHARACTERIZATION OF INFECTIOUS MYONECROSIS VIRUS (IMNV) ISOLATED FROM THE SHRIMP SPECIES Litopenaeus vannamei IN CEARÁ STATE, BRAZIL

Maria Verônyca Coelho Mello, Maria Izabel Florindo Guedes, Sergio Rodriguez Malaga, Lia Magalhães Almeida, Tatiane Rodrigues Oliveira


DOI: https://doi.org/10.3856/vol42-issue3-fulltext-22

Abstract


Litopenaeus vannamei shrimp, most important species in world aquaculture, has seriously affected by infectious myonecrosis virus (IMNV) that causes mortalities of up to 70%. With the aim of improving the development of new strategies for rapid and reliable diagnosis, we isolate IMNV, from L. vannamei farmed in Brazil, through a discontinuous sucrose gradient, and sequenced cDNA fragment encoding the major capsid protein from this virus. Nucleotides sequences corresponding to the viral capsid protein was obtained by RT-PCR and confirmed by automatic sequencing. Comparison with sequences which encode the capsid protein obtained from Indonesia isolates showed a high identity.

Mello M, Guedes M, Malaga S, Almeida L, Oliveira T. MOLECULAR CHARACTERIZATION OF INFECTIOUS MYONECROSIS VIRUS (IMNV) ISOLATED FROM THE SHRIMP SPECIES Litopenaeus vannamei IN CEARÁ STATE, BRAZIL. Lat. Am. J. Aquat. Res.. 2017;42(3). Available from: doi:10.3856/vol42-issue3-fulltext-22 [Accessed 22 Jan. 2019].
Mello, M., Guedes, M., Malaga, S., Almeida, L., & Oliveira, T. (2017). MOLECULAR CHARACTERIZATION OF INFECTIOUS MYONECROSIS VIRUS (IMNV) ISOLATED FROM THE SHRIMP SPECIES Litopenaeus vannamei IN CEARÁ STATE, BRAZIL. Latin American Journal of Aquatic Research, 42(3). doi:http://dx.doi.org/10.3856/vol42-issue3-fulltext-22