Influence of feeding on the circadian rhythm of digestive enzymes in cultivated juveniles of the freshwater crayfish Cherax quadricarinatus (Parastacidae)

Hernán J. Sacristán, Luis M. Franco-Tadic, Laura S. López-Greco


DOI: https://doi.org/10.3856/vol41-issue4-fulltext-12

We analyze in juvenile Cherax quadricarinatus fattening stage, the circadian rhythm of total protein levels, activity of proteases, lipases and amylases, and their possible change as a function of morning (8 h) and evening (17 h) feeding. In general, the activity of digestive enzymes of juvenile C. quadricarinatus showed no circadian pattern of secretion of lipases except where there was an increase towards the evening-night and a decrease towards the early hours of the morning. The total protein levels, the activity of proteinases and amylases of juveniles of C. quadricarinatus registered in the midgut gland remained virtually unchanged in all experiments. However, a non-significant tendency to diminish the proteinase activity levels after the feeding pulse was observed, with a return to previous enzymatic levels after 3 h. In contrast, the amylase activity did not show an observable effect by the feeding schedule. The study of the patterns of the digestive enzyme secretion and their possible changes, could be used as a tool to establish the most favorable times of the day for juveniles feeding.

Sacristán H, Franco-Tadic L, López-Greco L. Influence of feeding on the circadian rhythm of digestive enzymes in cultivated juveniles of the freshwater crayfish Cherax quadricarinatus (Parastacidae). Lat. Am. J. Aquat. Res.. 2017;41(4): 753-761. Available from: doi:10.3856/vol41-issue4-fulltext-12 [Accessed 4 Jun. 2020].
Sacristán, H., Franco-Tadic, L., & López-Greco, L. (2017). Influence of feeding on the circadian rhythm of digestive enzymes in cultivated juveniles of the freshwater crayfish Cherax quadricarinatus (Parastacidae). Latin American Journal of Aquatic Research, 41(4), 753-761. doi:http://dx.doi.org/10.3856/vol41-issue4-fulltext-12