Implementation, validation, and application of a new HPLC method to determine oxytetracycline in salmonid muscle tissue

Cristian Aguilera, Carlos Herrera, Jorge Ponce


DOI: https://doi.org/10.3856/vol38-issue2-fulltext-7

A method for identifying and quantifying oxytetracycline in salmon muscle, for quantifying oxytetracycline recovery levels, and for studying its pharmacokinetic in different organs of salmonids was implemented and validated. During implementation, antibiotic residues were detected and quantified in salmon muscle tissue. The principle of the HPLC methodology included determining a series of representative solutions of the standards, used to fortify the samples. The retention time was 5.7 min. The validation of the method sought to confirm and document the reliability of the results obtained with this method. For this, several parameters (selectivity, precision, accuracy, sensitivity, uncertainty) were analyzed. The results obtained indicated 96% recovery. The coefficient of correlation (R2) of the calibration curve was 0.9946 and the coefficient of variation was 1.86%.

Aguilera C, Herrera C, Ponce J. Implementation, validation, and application of a new HPLC method to determine oxytetracycline in salmonid muscle tissue. Lat. Am. J. Aquat. Res.. 2011;38(2): 227-233. Available from: doi:10.3856/vol38-issue2-fulltext-7 [Accessed 22 Apr. 2019].
Aguilera, C., Herrera, C., & Ponce, J. (2011). Implementation, validation, and application of a new HPLC method to determine oxytetracycline in salmonid muscle tissue. Latin American Journal of Aquatic Research, 38(2), 227-233. doi:http://dx.doi.org/10.3856/vol38-issue2-fulltext-7